Aberrant methylation of Glutathione Stransferase P1 and E-cadherin in invasive ductal breast carcinoma and fibroadenoma-中华乳腺病杂志（电子版）

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作者:: Wings;Tjing;Yung;;Loo;Mary;Ngan;Bing;Cheung;Louis;Wing;Cheong;Chow; UNIMED Medical Institute, Hong Kong(Wings TY Loo, Mary NB Cheung, Louis WC Chow); Organisation for Oncology and Translational Research Hong Kong (Wings TY Loo, Louis WC Chow); School of Chinese Medicine, The University of Hong Kong (Wings TY Loo); Clinica

关键词:: DNA hypermethylation; Methylation-specific PCR; Glutathione S-transferase P1 gene; E-cadherin; Breast invasive ductal carcinoma; Fibroadenoma

摘要:: Objective To investigate the hypermethylation status of glutathione transferase P1(GSTP1) and E-cadherin (ECAD), TSGs (tumor suppressor genes) in our breast cancer samples and explore their correlation with clinicopathological features of corresponding cancer patients. Methods One hundred and thirtysix IDC (invasive ductal carcinoma) patients were recruited for analysis and 16 fibroadenoma patients acted as control. DNA extraction and methylationspecific PCR (MSP) were subsequently performed preceded by pathological examination. Results The percentage of hypermethylated GSTP1 in carcinoma and fibroadenoma groups was 34.92% and 15.79% respectively and the percentage of hypermethylated ECAD in carcinomas and fibroadenomas was 18.00% and 0.00% respectively. Carcinoma had the highest percentage of cerbB2 overexpression being 54.55% among the clinicopathological parameters. Conclusion Hypermethylation patterns are frequent in IDC and seem to relate to c-erbB2 overexpression, and such epigenetic change should not be neglected in fibroadenoma. Tumor methylation status in cancer patients can be determined at early stage and it may be a reference for better treatment planning.