PEA3 activates CXCL12 transcription in MCF-7 breast cancer cells-中华乳腺病杂志（电子版）

文章信息/Info

作者:: CHEN;Li;CHEN;Bobin;LI;Junjie;JIN;Wei;SHAO;Zhimin; Department of Breast Surgery, Breast Cancer Institute, Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China（CHEN Li, LI Junjie, JIN Wei, SHAO Zhimin ）; Department of Hematology, Huashan Hospi

关键词:: polyomavirus enhancer activator 3 (PEA3); chemokine CXC motif ligand 12 (CXCL12) promoter; transcription regulation

摘要:: Objective To explore the activity of PEA3 (polyomavirus enhancer activator 3 ) on CXCL12 (Chemokine CXC motif ligand 12) transcription and to reveal the role of PEA3 involved in CXCL12mediated metastasis and angiogenesis in breast cancer. Methods Methods such as cell transfection, ChIP assay (chromatin immunoprecipitation ), and siRNA (small interfering RNA) were applied to demonstrate and confirm the interaction between PEA3 and CXCL12. Results Overexpression of PEA3 could increase the CXCL12 mRNA level and the CXCL12 promoter activity in human MCF7 breast cancer cells. ChIP assay demonstrated that PEA3 could bind to the CXCL12 promoter in the cells transfected with PEA3 expression vector. PEA3 siRNA decreased CXCL12 promoter activity and the binding of PEA3 to the CXCL12 promoter in MCF-7 cells.Conclusions PEA3 could activate CXCL12 promoter transcription. It may be a potential mechanism of tumor angiogenesis and metastasis regarding of PEA3 and CXCL12.