[1]Qing,Liu,等.Application of biomarkers in sentinel lymph node biopsy for the management of breast cancer[J].中华乳腺病杂志(电子版),2012,6(6):613-621.
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Application of biomarkers in sentinel lymph node biopsy for the management of breast cancer()

中华乳腺病杂志（电子版）[ISSN:1674-0807/CN:11-9146/R]

卷:
第6卷
期数:
2012年6期
页码:
613-621
栏目:
论著
出版日期:
2012-12-01

文章信息/Info

作者:
Qing;Liu; ;Wings;Tjing;Yung;Loo; ;Adrian;Yun;San;Yip; ;Louis;Wing;Cheong;Chow; Elizabeth;Lam;Yan;Ng; ;Mary;Ngan;Bing;Cheung
UNIMED Medical Institute, Hong Kong (Qing Liu, Wings Tjing Yung Loo, Louis Wing Cheong Chow, Mary Ngan Bing Cheung); Organization for Oncology and Translational Research, Hong Kong (Wings Tjing Yung Loo, Adrian Yun San Yip, Louis Wing Cheong Chow, Elizabe
关键词:
sentinel lymph node;  immunochemical staining;  cytokines;  biomarkers
摘要:
Objective Sentinel lymph node in breast cancer is a predictor of the tumor爷s metastatic potential. Cytokines emerging from antitumor immune response might also target sentinel lymph node (SLN). In this study, we evaluated the cytokine profile including interleukin-8 (IL-8), interleukin-12 (IL-12), interferon-gamma (IFN-γ), and tumor necrosis factor alpha (TNF-α) for the T-cell response. Methods　From January 2008 to August 2010,35 patients with breast mass underwent SLN biopsy in UNIMED Medical Institute. The specimens were formalin-fixed and paraffin-embedded for hematoxylin and eosin staining and immunohistochemistry with following markers: IL-8, IL-12, IFN-γand TNF-α. The staining intensity was scored according to four grades: negative (-), weak expression (+), moderately positive expression ( ++), strong positive expression ( +++). The correlation between the expression of cytokines and pathological characteristics were also analyzed using the Spearman Rank Correlation test. Results Out of 35 patients, SLN metastases were observed in 20 patients and among them, 75% (15/20) were strongly stained ( + + +) with IL-8 and moderately stained (++) with IL-12, but only 25% (5/20) were strongly stained (+++) with IFN-γand TNF-α. The expression of IL-8 was significantly associated with all tumor grades (r=0.639, P = 0.00), estrogen receptor (r = 0.778, P = 0.01), progesterone receptor (r =0.759 P = 0.00) and HER-2/ neu receptor (r = 0.863 P = 0.00). Conclusion　The strong association between IL-8 expression and SLN metastases might highlight the prognostic significance of IL-8 in SLN.
更新日期/Last Update: 2012-01-20