[1]Qing,Liu,等.Expression of cancer stem cell surface markers after chemotherapeutic drug treatment to reflect breast cancer cell regrowth[J].中华乳腺病杂志(电子版),2014,8(1):31-36.
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Expression of cancer stem cell surface markers after chemotherapeutic drug treatment to reflect breast cancer cell regrowth()

中华乳腺病杂志（电子版）[ISSN:1674-0807/CN:11-9146/R]

卷:
第8卷
期数:
2014年1期
页码:
31-36
栏目:
论著
出版日期:
2014-02-01

文章信息/Info

作者:
Qing;Liu; ;Wings;Tjing;Yung;Loo; ;Louis;Wing;Cheong;Chow; ;Kelly;Wei;Yu;Rui
UNIMED Medical Institute and Organization for Oncology and Translational Research, Hong Kong, China
关键词:
Neoplastic stem cells; Real-time polymerase chain reaction; Cell line;  tumor; Drug therapy;  Cell surface makers
摘要:
Objective To detect the cell viability and the expressions of stem cell surface markers after chemotherapeutic drug treatment. Methods We observed the cytotoxic effects of three chemotherapeutic agents [epirubicin (Epi), fluorouracil (5-FU) and cyclophosphamide (Cyc)] in three cell lines, and the cell
viabilities after removed these chemotherapeutic agents. Expressions of stem cell surface markers CD44, CD24, CD90, CD14 and aldehyde dehydrogenase1(ALDH1) in breast cancer cells were analyzed by real-time PCR. The post hoc analysis (Tukey's tests) in conjunction with one-way ANOVA was used for statistical analysis. Results The initial cytotoxic efficacy was most notable. After the treatment of the same therapeutic agents, cell viability was decreased by 64. 8% 35.14%, 32.25% in BT-483 cells, 66.4%, 22.94% and 45.88% in MDA-MB-231 cells, 97.1%, 99.5% and 76.4% in MCF cells. The difference was significant compared with that before treatment (P = 0.000). However, the inhibitory effects were diminished after chemotherapeutic agent withdrawal. Cell viabilities were increased to 167.9%, 212.04% and 188.66% in MDA-MB-231 cells at 48 h after withdrawal. At 72 h after withdrawal, cell viability was increased with a significant difference in three cell lines (all P values=0.000). Expressions of CD44 and ALDH1 were most prevalent for MDA-MB-231, BT-483 and MCF-7 cells. ALDH1 mRNA level was significant higher in BT-483 (HER-2 overexpression cell line) than MDA-MB-231 ( triple negative cell line) ( P = 0.012). CD14 mRNA level in MCF-7 cells were significantly lower than that in MDA-MB-231 and BT-483 (P = 0.003, 0.001). BT-483 showed significantly higher level of CD44 than MDA-MB-231 and MCF-7 cell line (P = 0.013, 0.020), and no significant difference was detected between MDA-MB-231 and MCF-7 breast cancer cells (P = 0.955). CD90 mRNA expressions were detected in MDA-MB-231 cells and MCF-7 cells, but not in BT-483 cells. Conclusion Some malignant cells could survive in vitro and begin to proliferate again between cycles of chemotherapy.
更新日期/Last Update: 2014-01-20