[1]Wings,T.,Y.,等.Application of single and combination therapy of clarithromycin and tamoxifen to suppress breast cancer cell proliferation and metabolism[J].中华乳腺病杂志(电子版),2012,6(1):45-53.
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Application of single and combination therapy of clarithromycin and tamoxifen to suppress breast cancer cell proliferation and metabolism()

中华乳腺病杂志（电子版）[ISSN:1674-0807/CN:11-9146/R]

卷:
第6卷
期数:
2012年1期
页码:
45-53
栏目:
论著
出版日期:
2012-02-01

文章信息/Info

作者:
Wings;T.;Y.;Loo; ;Louis;W.;C.;Chow; ;Adrian;Y.;S;Yip;and;Mary;N.;B.;Cheung
UNIMED Medical Institute, Hong Kong (Wings T. Y. Loo, Louis W. C. Chow and Mary N. B. Cheung);Organization for Oncology and Translational Research, Hong Kong (Wings T. Y. Loo, Louis W. C. Chow and Adrian Y. S Yip);School of Chinese Medicine,Li Ka Shing Fa
关键词:
clarithromycin; tamoxifen; breast neoplasms; metabolism; cell proliferation
摘要:
Objective This study compares the anti-tumor effects of single and combination use of clarithromycin and tamoxifen on estrogen receptor (ER) positive breast cancer cell lines, BT-483 and MCF-7 as well as triple negative cell line, MBA-MD-231, which acts as a negative control. The effect of solid breast tumor inhibition by clarithromycin is also studied. Method BT-483, MCF-7 and MBA-MD-231 were cultured in 6-well plates in a 37 益humidified incubator without CO2 for 24 h prior to the addition of the test drugs. The test groups were clarithromycin (Group 1), tamoxifen (Group 2), clarithromycin and tamoxifen (Group 3), and control (Group 4). Group 3 was prepared in 1 to 1 ratio at a concentration of 1. 5 mmol/ L clarithromycin and 25 滋mol/ L tamoxifen. On the other hand, 1 mm3 solid breast tumors were submerged into various groups as above for 24 h. On the harvest day, the proliferation of cancer cells and solid breast tumor samples were measured by WST-1 proliferation reagent while ATP bioluminescence assay was employed to measure the metabolic rate of the three cell lines. Results The proliferation of BT-483 and MCF-7 was suppressed most by combination use of clarithromycin and tamoxifen with statistical significance. The two drugs did not have an inhibitory effect on the hormonal negative cancer cells. For solid breast tumor samples, all the test groups showed reduced metabolic rate as compared with the control group (P <0.05). Conclusion Combination use of tamoxifen and clarithromycin are effective in suppressing cell proliferation and metabolism rate of breast cancer cells while single use of clarithromycin effectively inhibits the proliferation of solid breast tumor.
更新日期/Last Update: 2012-01-20